10x faster single cell force spectroscopy
with the FluidFM ADD-ON for AFM
FluidFM-based single cell force spectroscopy overcomes the limits of existing methods.
How can our technology help your research?
Pick a cell with negative pressure, measure, release it again with a positive pressure pulse or by a short cleaning procedure.
FluidFM-based single cell force spectroscopy.
10x faster. 10x higher force range. 20 to 200 cells a day.
Get sound statistics in one day instead of weeks or months - with the FluidFM ADD-ON for your AFM.
Both in biophysics and mechanobiology, the physical study of single cells allows insights into biological phenomenon such as differentiation, growth and proliferation. In cancer research, stem cells, and organoids, the cell mechanical properties and interactions with its environment are key to obtain a deeper understanding. Further, for implant materials, there is a clinical need to understand and control how various cells adhere to it.
With each cell being different from its neighbor – also known as cell heterogeneity - one goal is to understand these effects on a single cell level. Single cell force spectroscopy has been established as an insightful method to address such questions using atomic force microscopy (AFM).
Compared to conventional methods, FluidFM offers a dramatic improvement by reversibly immobilizing a cell to a FluidFM probe by suction, and subsequent release with pressure. This allows an increase of the throughput by a factor of 10 to 100.
Get sound statistics within one day instead of weeks or months.
FluidFM has provided us the chance to detach mammalian cells that were very strongly adhered to the substrate, in a systematic way and without any chemical modification of the cantilever; thus, allowing us to study cell behaviour in their natural state and environment.
Publication highlights using FluidFM for single cell force spectroscopy
Thanks to the unique properties of FluidFM technology, you can gather solid cell mechanical data in a much shorter time. Gain access to unparalleled measurement ranges, increasing your experimental flexibility. FluidFM gives you the edge.
Millions of people suffer from Leukemia around the world. While treatment drugs exist, cancer regularly develops a resistance against them. Researchers from both the University Hospital Würzburg and the University Würzburg found potentially a new approach to overcome resistance to the recently approved Midostaurin drug and even increase the drug's activity, with help of FluidFM cell measurements.
Stents help millions of people every year to overcome arterial blockages, saving many lives in the process. Once implanted, the stent should integrate well and prevent formation of blood clots. In this publication research groups from ETH Zurich investigate stent design optimization by measuring cell adhesion to its surface with FluidFM.
Calibrating high-throughput devices
Single cell force spectroscopy offers fundamental insights into many fields yet suffers from low throughput. MTA Budapest tremendously speeds up the acquisition of single cell adhesion data by using FluidFM adhesion measurements to calibrate an optical sensor array. Thus, they are able mechanically monitor more than 1000 adherent cells in parallel.
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