FluidFM® ADD-ON for your
Nanosurf AFM System
POWERFUL & AFFORDABLE UPGRADE SOLUTION
Empower your Nanosurf AFM beyond imaging.
The FluidFM ADD-ON greatly extends the application scope of classical atomic force microscopes (AFMs) beyond imaging and force spectroscopy. From single cell biology to material sciences – benefit from more than 8 FluidFM applications using the AFM you already own.
The FluidFM ADD-ON is available for the Nanosurf FlexAFM and CoreAFM platforms and a unique integrated FPM solution on the FlexAFM
Fully integrated system with user-friendly FluidFM® ARYA operator software
Integrated solution with optical, force, and fluidic control
Predefined experimental workflows
Conduct original research at the frontiers of science
8+ FluidFM applications
Single cell / microbe force spectroscopy
Main advantages of FluidFM:
For bacteria/microbe and suspended cell adhesion
Higher cell-substrate adhesion forces can be measured — one order of magnitude
Longer cell-substrate interaction times can be studied — orders of magnitude
Much shorter measurement time / Many more adhesion measurements — factor 20
Study of tumor progression and metastasis with cell-cell adhesion forces
Study with a Flex-FPM by Dr. Noa Cohen, group of Prof. Tanya Konry, Northeastern University in Boston, on cell-cell adhesion forces to gain more insights into tumor progression and metastasis (Cohen et al., 2017):
Optical images showing A) a single cell to be picked up by a FluidFM probe B) the cell aspired to the cantilever and C) the FluidFM probe with aspired cell during a cell-cell adhesion measurement. Data/image courtesy of Tanya Konry group, Northeastern University, Boston, USA.
A) Typical force curves between a MCF7 cell aspried to the cantilever and non-cancerous, firbroblast (HS5) on the substrate at different contact times. B) Development of the force with contact time between the cells. Data/image courtesy of Tanya Konry group, Northeastern University, Boston, USA.
FluidFM probes allow to pick up colloids from sub micrometer to 100 micrometer in diameter, either to quantify mechanical properties of a substrate or to study the colloid itself. The colloid can be replaced within minutes once it is dirty or a different size/functionality is desired.
Main advantages of FluidFM:
Handling — Pre-coated beads and easy mounting
Experiment design — Same cantilever and different beads
Time — Fast mounting and bead exchange
Easy — Intuitive touchscreen-based workflow environment
Colloidal spectroscopy of HeLa cells using FluidFM
A colloid is picked up by FluidFM and used to probe a living cell and create a force map. The FluidFM microfluidic control is fully integrated into the AFM software.
Left image: Map of Young's modulus (blue area is on glass.
Right image: Approach curves (force in nN versus tip-sample distance in µm) for the locations indicted with a star on the left: on glass (blue), harder cell areas (orange) and softer cell areas (black).
Image courtesy of Nanosurf AG.
Spotting & lithography
Deposit (bio)molecules and particles at defined locations with micrometer accuracy and with femtoliter volumes. A wide range of inks can be deposited in both air and liquid environment - from water to honey, solvents, acids, bases and more. For nanoscale and biomedicine research, bio-sensors and biomaterials.
Creating grids & patterns
Automatically varying back pressure and contac time in a grid-like pattern (3 dots per condition), spot sizes can be quickly optimized. Image courtesy of Nanosurf AG.
Nanosurf logo written in air with a solution containing approximately 50% glycerol; back pressure 200 mbar. Image courtesy of Nanosurf AG.
Single cell injection
Introduce any payload into the cell of choice.
Typical injected volumes range from a few dozen to 200 fL, a small fraction of the overall cell volume of 4 to 5 pL. If needed the injected volume can be quantified with fL precision for each injected cell with a fluorescence intensity based approach.
The video shows a FluidFM injection of Lucifer Yellow, a fluorescent tracer, into CHO cells with CoreAFM DIMO (courtesy of Nanosurf AG) . It is common that any payload e.g. CRISPR is co-injected with a fluorescent tracer to have a live feedback on both the injection success and the injected volume.
How the FluidFM ADD-ON brings even more "Bio" into BioAFMs.
Innovative and intuitive handling
This video shows how a FluidFM probe is filled and mounted onto a Nanosurf FlexAFM (courtesy of Nanosurf AG).
FluidFM probes enable all FluidFM applications, and are tailored to the geometrical and physical requirements of the specific AFM.
The custom FluidFM probe holder for your AFM assures safe, tight and reliable mounting of FluidFM probes. (More images in the gallery.)
For more information visit nanosurf.com.
EMPOWER YOUR AFM RESEARCH WITH 8+ FluidFM APPLICATIONS.