Spotting of 2D viral structures by FluidFM

Digital FluidFM Conference 2021

Spotting of 2D viral structures by FluidFM

Proposed by Christine Müller-Renno

Nanotechnology plays an important role in different areas of application. Because top-down based fabrication technologies are restricted by physical and technical limits, bottom-up based fabrication technologies are of increasing interest. In this context, small building blocks are used to build larger objects through self-assembly. Because of their size and properties, plant viruses are ideal building blocks. Here, tomato bushy stunt viruses (TBSV), icosahedral plant viruses of 30 nm diameter, were used. TBSV can be genetically modified to create new properties or functionalities. Up to now, we have used TBSV with different side chains (aspartic (D), arginine (R), or histidine (H) tags) to build 2D and 3D layer systems through self-assembly [1, 2, 3]. In the future, real 3D structures, e.g. for building 3D nanoobjects or nanomachines, are in our focus for which defined 2D patterns such as dots, rings, or squares are required. These defined 2D patterns can then serve as a basis for the real 3D structures. To create such 2D patterns, the FluidFM® was used. For this purpose, viral structures were created by filling the FluidFM® probe (nanopipette/nanosyringe) with a solution containing 4D6H-TBSV. By applying certain pressure to the FluidFM® probe, different dots containing viral nanoparticles are placed on the substrate.

FluidFM® spotting was optimized by systematically varying parameters such as virus concentration, overpressure, and setpoint force. The quality of the individual viral patterns was examined using the Scanning Force Microscope (SFM) [4]. Important parameters to be controlled are on the one hand properties of the solution like viscosity and surface tension, on the other hand, shape, size, and concentration of the nanoparticles, here the virus particles. These parameters do not only determine the droplet formation and virus attachment to the surface but, together with the properties of the ambient like humidity and temperature, the movement of the particles during the drying process. Here, the Marangoni effect and the Deegan flow together with the other parameters define whether a coffee ring pattern with or without a central dot or a uniform distribution of the nanoparticles is observed. Last but not least the used type of FluidFM® probe and herewith the geometry of the contact between aperture and substrate are playing an important role.

[1] A. Lüders, C. Müller, K. Boonrod, G. Krczal, C. Ziegler, Colloids and Surfaces B: Biointerfaces 2012, 91, 154.
[2] V. Rink, C. Müller-Renno, C. Ziegler, M. Braun, K. Boonrod, G. Krczal, Biointerphases 2017, 12, 05G606.
[3] C. Müller-Renno, V. Rink, M. Ani, M. Braun, K. Boonrod, G. Krczal, Ch. Ziegler, Biointerphases 2020, 15, 041009.
[4] C. Müller-Renno, D. Remmel, M. Braun, K. Boonrod, G. Krczal, Ch. Ziegler. Producing plant virus patterns with defined 2D structure. Physica Status Solidi A 2021, 2100259.

C. Müller-Renno1, D. Remmel1, M.Braun2, K. Boonrood2, G. Krczal2, Ch. Ziegler1
1
Department of Physics and Research Center OPTIMAS, TU Kaiserslautern, Germany
2RLP Agroscience GmbH, Neustadt/Weinstraße, Germany
Corresponding author: cz@physik.uni-kl.de


TU Kaiserslautern (AG Ziegler), Christine Müller-Renno

Christine Müller-Renno

Senior Scientist / Gruppenleiterin Biophysik, Department of Physics, TU Kaiserslautern, Germany



Practical Info

Date
Dec 01, 2021 09:35 AM (Europe/Zurich)
Duration
20 minutes
Location
Digital