- Pablo Doerig
- Dec 01, 2021 09:00 AM
Welcome note. Conference introduction. Advances and novelties offered by FluidFM technology and solutions....
- Gang-Yu Liu
- Dec 01, 2021 09:15 AM
Combining microfluidic probe with atomic force microscopy (AFM) developed by Cytosurge AG, researchers are able to probe and investigate chemical and biological events occurring at single cell level. Single cell mechanics, derived from atomic force microscopy-based technology in conjunction with cellular imaging and assays, provides a new and effective means to measure the elastic compliance of cellular membrane, cytoskeleton, as well as nuclei. In addition, applying mechanical force to living...
- Christine Müller-Renno
- Dec 01, 2021 09:35 AM
Nanotechnology plays an important role in different areas of application. Because top-down based fabrication technologies are restricted by physical and technical limits, bottom-up based fabrication technologies are of increasing interest. In this context, small building blocks are used to build larger objects through self-assembly. Because of their size and properties, plant viruses are ideal building blocks. Here, tomato bushy stunt viruses (TBSV), icosahedral plant viruses of 30 nm diameter, ...
- Dec 01, 2021 09:55 AM
- Kinga Dóra Kovács
- Dec 01, 2021 10:05 AM
The present talk shortly introduces the main research activity of the Nanobiosensorics Laboratory (Budapest, Hungary, https://nanobiosensorics.com) especially in relation to fluidic force microscopy. We mainly focus on label-free techniques for cell biological and biophysical research and on developing kinetic biophysical models (Kanyo et al., 2020; Saftics et al., 2021). Recently by employing resonant waveguide gratings, we could demonstrate single-cell resolution and measure the adhesion kinet...
Probing the interactions between air bubbles and (bio)-interfaces at the molecular scale using FluidFM
- Cécile Formosa-Dague
- Dec 01, 2021 10:25 AM
Understanding the molecular mechanisms underlying bubble-(bio)surfaces interactions is currently a challenge that if overcame, would allow to understand and control the various processes in which they are involved. Atomic force microscopy is a valuable tool to measure such interactions, but it is limited by the large size and instability of bubbles that can be attached on surfaces or on AFM cantilevers. To overcome these challenges, we here develop a new method to probe more accurately the inter...
4D force detection of cell adhesion and contractility combining FluidFM and confocal reference-free TFM
- Xinyu Zhang
- Dec 01, 2021 10:45 AM
Fluid Force Microscopy (FluidFM) combines a hollow microchannel cantilever with conventional AFM and has proven to be a promising tool in biological applications, especially in cell mechanics.  FluidFM and Atomic Force Microscopy (AFM) provide abundant force sensitivity in the perpendicular direction but lack information in the xy lateral direction. Confocal reference-free Traction force microscopy (cTFM) is a powerful tool for the quantification of cellular forces and allows the continuous h...
- Dec 01, 2021 11:05 AM
- Hans Gunstheimer
- Dec 01, 2021 11:15 AM
In this presentation we will discuss mass measurements by FluidFM ® Micropipettes, their accuracy and limits illustrated with examples from biology and material science. Mass as a fundamental property gives us valuable information about the condition of a measurement object. Taking a single biological cell for example, the dysregulation of its mass and its mass development can be related to many diseases (1). To relate the mass of the cell to its condition it is favorable to combine it with othe...
- Alice Battistella
- Dec 01, 2021 11:35 AM
In mammalian fertilization process, one, and only one, spermatozoon must cross the glycoprotein thick protective shell, the zona pellucida (ZP) and enter the oocyte to initiate the meiotic division. We recently demonstrated that the mechanics of this layer plays an important role in the process and show that during ageing the ZP nearly double its stiffness thus reducing the probability of fertilization (Battistella 2021). This suggests that, beside the enzymatic mechanism, also the mechanical in...
- Pablo Doerig
- Dec 01, 2021 11:55 AM
- Dec 01, 2021 02:30 PM
Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in single cell omics....
- Dec 01, 2021 04:00 PM
Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in nanoprinting....
- Dec 02, 2021 09:00 AM
Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in neuroscience....
- Dec 02, 2021 10:30 AM
Interactive round table to discuss approaches, ideas, and solutions related to FluidFM research in mechanobiology....