Watch the presentation from Dr. Paul Monnier:
FluidFM: A new approach to CRISPR editing
Works with any cargo &
adherent cell type
For cargos such as mRNA, CRISPR and gRNA. Ideal for rare and hard-to-transfect cells.
Easy generation of clones in < 3 weeks with one instrument
1. Direct intra-nuclear delivery 2. Isolation of specific cells directly from the culture 3. Expansion of the isolated cell
& stack editing
Deliver 100's of different gRNAs at the same time. Inject different cocktails at different time points.
Listen to the well received talk from Dr. Paul Monnier, Field Application Scientist on how our patented hollow probe FluidFM technology could significantly improve CRISPR research:
A presentation from Genome Editing Congress in Boston showing you how to overcome with FluidFM technology one of the biggest challenges in gene editing: direct delivery of reagents into the nucleus.
Use Case #1
Generation of multiple KO clones. One instrument, < 3 weeks.
Injection of multiple gRNAs & Cas9
Multiple gRNAs are simultaneously delivered with the Cas9 into CHO cells. A fluorescent marker is co-injected to verify successful delivery.
Hands-on time: 30 minutes - from the loading of the FluidFM Nanosyringe until the injection of 50 cells is completed
Isolation of the clone candidate
Selected cells are isolated into separate wells. Visual proof of single cell deposition ensures monoclonality.
Hands-on time: 30 minutes - for the preparation of the FluidFM Micropipette and the isolation of 12 injected cells expressing the fluorescent marker
As of day 3:
Clone expansion and analysis
Of the isolated clones, 90% developed into monoclonal colonies and 50% thereof showed mutations in targeted loci.
Strong reduction in complexity and development time of genetically modified cell lines starting from few nano-injected cells
Use Case #2
Successful transfection of primary neurons with plasmids
With our system, even primary neurons can be transfected successfully and highly efficiently. The FluidFM nanosyringe directly delivers the complexes into primary neurons - gently and autonomously.
Conventional delivery methods applied to neurons are often inefficient, expensive and toxic to the neuron.
GFP expression in neurons 24h after nano-injection of plasmids in a mouse primary neuron. Courtesy of Jinan University Guangzhou, China.
Use Case #3
Transient expression of target proteins with mRNA
Human Dermal Fibroblast injected with GFP mRNA. 70% of injected cells expressed GFP 24h after injection.
Human dermal fibroblast expressing GFP, 24h after injection.
Transient mRNA transfection helps to give fundamental insights on protein functions at a single cell level, such as protein expression and intercellular communication.
mRNA transfection using FluidFM nano-injection results in:
Higher transfection efficiency
Lower cell toxicity
Use Case #4
Straight-forward multiplex & stack editing
A CHO cell was injected at four different time points . Lucifer Yellow was used to calculate after each injection the delivered volume.
Four serial injections into the same cell.
Multiplex and stack editing with FluidFM overcomes the limitations of conventional methods:
Full freedom in designing the injected cocktail: a nano-syringe can be loaded with 100's of different gRNAs. Biology is the limit.
Inject different cocktails at different time points: >95% of cells survive an injection procedure.
Interested to know more about our CRISPR solution?
Find out more on how our technology can help your research.