Application Note

FluidFM nano-injection overcomes delivery limitations of current CRISPR gene editing methods, accelerates cell line development cycles, and is poised to significantly broaden multiplexing capabilities 

Paul Monnier 2 , Jennifer Rottenberger 1 , Maria Milla2, Tobias Beyer 2 , Dario Ossola 2 , Justin S Antony 1  and Markus Mezger 1
1) University Children's Hospital, Department of Pediatrics I, Hematology and Oncology, University of Tübingen, Tübingen, Germany 
2) Cytosurge AG, Saegereistrasse 25, 8152 Glattbrugg, Switzerland

Pharmaceutical and biological research as well as biologics manufacturing rely on genetically modified cell lines with genes that have been modified to induce the desired phenotype. With the discovery and development of gene editing technologies like CRISPR, the potential of doing multi-loci edits has received much interest but has proven to be a tedious and long process. Here we demonstrate the generation of a monoclonal multiple Knock-Out cell line in less than three weeks with the help of the FluidFM technology.

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FluidFM Cell Line Development workflow. Day 1, cells are transfected via FluidFM nano-injection. On day 2, successfully transfected cells are selected and isolated via FluidFM pick and place. From day 3 to 14+, isolated single cells expand into a stable monoclonal cell line and their genome is analyzed. 

Boost CRISPR efficiency by direct intra-nuclear delivery

Fast & easy multiplex genome editing, optimization of off-target effects, ...

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